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2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador
PLOS Neglected Tropical Diseases, 1: 1-21 (2017)
Investigador(es) PUCE Costales Cordero, Jaime Alfredo; Ocaña Mayorga, Sofía Beatriz; Villacis Salazar, Anita Gabriela; Yumiseva Marín, César Alberto Autores Luis E. Hernandez-Castro, Marta Paterno, Anita G. Villacís, Björn Andersson, Jaime A. Costales, Michele De Noia, Sofía Ocaña-Mayorga, Cesar A. Yumiseva, Mario J. Grijalva y Martin S. Llewellyn
Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru. Genomic approaches and next generation sequencing technologies have become powerful tools for investigating population diversity and structure which is a key consideration for vector control. Here we assess the effectiveness of three different 2b restriction site-associated DNA (2b-RAD) genotyping strategies in R. ecuadoriensis to provide sufficient genomic resolution to tease apart microevolutionary processes and undertake some pilot population genomic analyses.
